Growth of Transplastomic Cells Expressing d-Amino Acid Oxidase in Chloroplasts Is Tolerant to d-Alanine and Inhibited by d-Valine

Growth of transplastomic cells expressing D-amino acid oxidase in chloroplasts is tolerant to D-alanine and inhibited by D-valine.

Dual-conditional positive/negative selection markers are versatile genetic tools for manipulating genomes. Plastid genomes are relatively small and conserved DNA molecules that can be manipulated precisely by homologous recombination. High-yield expression of recombinant products and maternal inheritance of plastid-encoded traits make plastids attractive sites for modification. Here, we describ...

Biological role of D-amino acid oxidase and D-aspartate oxidase. Effects of D-amino acids.

D-Amino acids administered to animals are absorbed by the intestine and transported through the blood-stream to solid tissues where they are oxidized in vivo by D-amino acid oxidase and D-aspartate oxidase to produce the same compounds they do in vitro; i.e. NH3, H2O2, and the keto acid corresponding to the amino acid ingested. In the liver and kidneys of the animals, an inverse relationship ex...

Intestinal bacterial origin of D-alanine in urine of mutant mice lacking D-amino-acid oxidase.

Urine from mutant mice which lack D-amino-acid oxidase contained a large amount of D-alanine. The alanine content was not changed by changes in the dietary composition or by starvation. However, oral administration of an antibiotic, amoxicillin, decreased the urinary alanine to the normal level. These results suggest that the D-alanine is not of dietary origin but of intestinal bacterial origin.

On the reaction of D-amino acid oxidase with -chloro-D-alanine.

The reactions of D-amino acid oxidase with /?-chloro-D-alanine and /?-chloro-L-alanine have been described. Both the holoenzyme and the apoenzyme catalyse the deamination of /?-chloro-L-alanine to keto acid without any consumption of oxygen or oxidation-reduction of the enzyme-bound FAD. The anaerobic reaction of the holoenzyme with yS-chloro-D-alanine involves three phases with respect to the ...

Astudyon D-amino-acid Oxidase